4WO7
Crystal Structure of PrsA from Bacillus subtilis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-07-13 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 1.36246 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 69.290, 87.680, 234.100 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 54.364 - 2.630 |
R-factor | 0.2275 |
Rwork | 0.226 |
R-free | 0.25120 |
Structure solution method | SAD |
RMSD bond length | 0.005 |
RMSD bond angle | 1.040 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHENIX |
Refinement software | PHENIX ((phenix.refine: dev_1779)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 117.000 | 2.790 |
High resolution limit [Å] | 2.630 | 2.630 |
Rmerge | 0.054 | |
Number of reflections | 21428 | |
<I/σ(I)> | 19.7 | 1.6 |
Completeness [%] | 98.8 | 90.2 |
Redundancy | 6.7 | 5.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 303.15 | 17 % PEG2000MME, 0.1M Hepes pH 7.5 |