4WNR
Structure of methanosarcina barkeri Roco2 RocCORdC bound to GDP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-11-10 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.06997 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 109.360, 109.360, 223.100 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.060 - 2.900 |
| R-factor | 0.24169 |
| Rwork | 0.240 |
| R-free | 0.26867 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3dpu |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.769 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.000 | 3.080 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Number of reflections | 18092 | |
| <I/σ(I)> | 21.84 | 2.84 |
| Completeness [%] | 99.6 | 98.6 |
| Redundancy | 8 | 8.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293.15 | Tris, Sodiumsulfate, Lithiumsulfate, PEG 400 |
