4WNN
SPT16-H2A-H2B FACT HISTONE Complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL7-1 |
Synchrotron site | SSRL |
Beamline | BL7-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-12-06 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.127090 |
Spacegroup name | H 3 |
Unit cell lengths | 109.667, 109.667, 187.470 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 21.220 - 1.800 |
R-factor | 0.181 |
Rwork | 0.179 |
R-free | 0.22500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1id3 |
RMSD bond length | 0.007 |
RMSD bond angle | 0.952 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 1.860 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.086 | 0.928 |
Number of reflections | 77661 | |
<I/σ(I)> | 20.2 | |
Completeness [%] | 98.7 | 95.6 |
Redundancy | 3.7 | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 277 | Crystals of ~ 60 x 60 mm formed within 10 days in 0.1M SPG (succinic acid:sodium dihydrogen phosphate:glycine ) pH 7, 25 % PEG 1500 (A4 of the PACT suite commercial screen (Qiagen). |