4WNF
Crystal structure of the oxidized TPR domain of LGN in complex with Frmpd4/Preso1 at 2.9 Angstrom resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL44XU |
Synchrotron site | SPring-8 |
Beamline | BL44XU |
Temperature [K] | 90 |
Detector technology | CCD |
Collection date | 2013-12-12 |
Detector | RAYONIX MX300HE |
Wavelength(s) | 0.9000 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 92.599, 92.599, 175.238 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 39.120 - 2.900 |
R-factor | 0.2391 |
Rwork | 0.237 |
R-free | 0.28020 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4wne |
RMSD bond length | 0.009 |
RMSD bond angle | 1.138 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP (11.0.05) |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.950 |
High resolution limit [Å] | 2.900 | 7.860 | 2.900 |
Rmerge | 0.075 | 0.022 | 0.502 |
Total number of observations | 74336 | ||
Number of reflections | 10385 | ||
<I/σ(I)> | 15 | 2.728 | |
Completeness [%] | 99.3 | 91.6 | 100 |
Redundancy | 7.2 | 6.1 | 7.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 0.1 M BisTrispropane (pH 7.5), 0.2 M potassium thiocyanate, 20% PEG 3350 |