4WNA
Structure of the Nitrogenase MoFe Protein from Azotobacter vinelandii Pressurized with Xenon
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2001-11-14 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.5418 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 77.123, 129.780, 107.543 |
Unit cell angles | 90.00, 108.94, 90.00 |
Refinement procedure
Resolution | 101.720 - 2.000 |
R-factor | 0.16985 |
Rwork | 0.167 |
R-free | 0.21869 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.291 |
Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 101.720 |
High resolution limit [Å] | 2.000 |
Number of reflections | 133045 |
<I/σ(I)> | 9.2 |
Completeness [%] | 98.7 |
Redundancy | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 297 | PEG 6000, sodium chloride, xylitol, imidazole/malate, spermine, Zwittergent |