4WN9
Structure of the Nitrogenase MoFe Protein from Clostridium pasteurianum Pressurized with Xenon
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-2 |
Synchrotron site | SSRL |
Beamline | BL12-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-08-02 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9537 |
Spacegroup name | P 1 |
Unit cell lengths | 67.309, 73.446, 108.658 |
Unit cell angles | 73.47, 87.56, 83.98 |
Refinement procedure
Resolution | 39.750 - 1.900 |
R-factor | 0.18215 |
Rwork | 0.179 |
R-free | 0.23427 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.346 |
Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 39.750 |
High resolution limit [Å] | 1.900 |
Number of reflections | 137866 |
<I/σ(I)> | 16 |
Completeness [%] | 96.7 |
Redundancy | 3.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 297 | PEG 3350, lithium citrate, sodium dithionite |