4WN0

Xenopus laevis embryonic epidermal lectin in complex with glycerol phosphate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-G
Synchrotron site	APS
Beamline	21-ID-G
Temperature [K]	100
Detector technology	CCD
Collection date	2014-08-01
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.97856
Spacegroup name	P 6
Unit cell lengths	124.628, 124.628, 55.585
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.935 - 2.200
R-factor	0.1524
Rwork	0.151
R-free	0.16920
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.089
Data scaling software	SCALEPACK
Phasing software	PHASER (2.5.2)
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.400	30.400	2.280
High resolution limit [Å]	2.200	4.730	2.200
Rmerge		0.048	0.755
Rmeas	0.133	0.051	0.792
Rpim	0.040	0.016	0.237
Total number of observations	285681
Number of reflections	25366
<I/σ(I)>	5.9
Completeness [%]	100.0	100	100
Redundancy	11.3	10.6	11.1
CC(1/2)		0.998	0.866

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	298	100 mM Tris-HCl, 20-24% PEG 400