4WML
Crystal structure of Saccharomyces cerevisiae OMP synthase in complex with PRP(CH2)P
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | MAX II BEAMLINE I911-3 |
Synchrotron site | MAX II |
Beamline | I911-3 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-11-15 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1.000 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 61.560, 61.560, 132.490 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 31.000 - 1.730 |
R-factor | 0.21335 |
Rwork | 0.211 |
R-free | 0.25017 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2pry |
RMSD bond length | 0.019 |
RMSD bond angle | 1.984 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 33.140 | 1.770 |
High resolution limit [Å] | 1.730 | 1.730 |
Number of reflections | 26807 | |
<I/σ(I)> | 28.63 | 1.45 |
Completeness [%] | 97.4 | 100 |
Redundancy | 13.5 | 10.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 295 | 0.1 M NaHEPES pH 7.5, 10 % Isopropanol, 20 % PEG 4000 |