4WMH
Structure of heme oxygenase-2 containing residues 1-288 lacking the membrane spanning region
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-08-18 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97857 |
| Spacegroup name | P 43 2 2 |
| Unit cell lengths | 35.690, 35.690, 366.706 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 91.680 - 2.500 |
| R-factor | 0.2308 |
| Rwork | 0.226 |
| R-free | 0.26990 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2qpp |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.947 |
| Data reduction software | XDS |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 91.680 |
| High resolution limit [Å] | 2.500 |
| Number of reflections | 8270 |
| <I/σ(I)> | 17.36 |
| Completeness [%] | 99.8 |
| Redundancy | 25.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 20% PEG 1.5K and 100mM BTP pH 7.0 |
