4WMG

Structure of hen egg-white lysozyme from a microfludic harvesting device using synchrotron radiation (2.5A)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	294
Detector technology	PIXEL
Collection date	2014-04-09
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.98
Spacegroup name	P 43 21 2
Unit cell lengths	79.250, 79.250, 37.970
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	22.550 - 2.500
R-factor	0.275
Rwork	0.274
R-free	0.30600
RMSD bond length	0.005
RMSD bond angle	0.978
Refinement software	PHENIX ((PHENIX.REFINE: DEV_1760))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	22.600	2.340
High resolution limit [Å]	2.300	2.300
Number of reflections	4696
<I/σ(I)>	8
Completeness [%]	81.5	55.8
Redundancy	4.4	1.67

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	BATCH MODE	3.5	294	15% sodium chloride, 6% poly-ethylene glycol 8000 and 0.5M sodium acetate