4WM8
Crystal Structure of Human Enterovirus D68
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 14-BM-C |
Synchrotron site | APS |
Beamline | 14-BM-C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-06-13 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9787 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 327.000, 347.800, 357.100 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.250 - 2.000 |
Rwork | 0.275 |
R-free | 0.27900 |
RMSD bond length | 0.006 |
RMSD bond angle | 1.400 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | HKL-3000 |
Refinement software | CNS (1.3) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.070 |
High resolution limit [Å] | 2.000 | 4.310 | 2.000 |
Rmerge | 0.105 | 0.061 | 0.754 |
Rmeas | 0.131 | 0.074 | 0.951 |
Rpim | 0.077 | 0.040 | 0.571 |
Total number of observations | 1586943 | ||
Number of reflections | 748623 | ||
<I/σ(I)> | 9.3 | ||
Completeness [%] | 55.7 | 52.5 | 41.6 |
Redundancy | 2.1 | 2.5 | 2 |
CC(1/2) | 0.992 | 0.654 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | 0.1 M Sodium Acetate (pH 4.5), 3.5 M Sodium Formate |