4WLZ
Crystal structure of mouse Xyloside xylosyltransferase 1 complexed with manganese and UDP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-04-17 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.1000 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 89.662, 89.662, 154.987 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 3.030 |
| R-factor | 0.23309 |
| Rwork | 0.230 |
| R-free | 0.28816 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.962 |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.140 |
| High resolution limit [Å] | 3.030 | 3.030 |
| Rmerge | 0.130 | 0.547 |
| Number of reflections | 14615 | |
| <I/σ(I)> | 13.7 | 2.7 |
| Completeness [%] | 99.3 | 95 |
| Redundancy | 9 | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 20 mM HEPES, 1.5 M Li2SO4 |
