4WLX

High pressure protein crystallography of hen egg white lysozyme at 280 MPa

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NW12A
Synchrotron site	Photon Factory
Beamline	AR-NW12A
Temperature [K]	298
Detector technology	CCD
Collection date	2013-12-01
Detector	ADSC QUANTUM 210r
Wavelength(s)	0.75
Spacegroup name	P 43 21 2
Unit cell lengths	78.023, 78.023, 38.031
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.210 - 1.600
R-factor	0.15381
Rwork	0.152
R-free	0.19306
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.024
RMSD bond angle	2.146
Refinement software	REFMAC (5.7.0032)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.600
Number of reflections	16058
<I/σ(I)>	51.5
Completeness [%]	99.6
Redundancy	6.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	BATCH MODE		293	Sodium chloride