4WLM
Crystal structure of mouse Xyloside xylosyltransferase 1 complexed with manganese
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-09-08 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.1000 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 89.306, 89.306, 154.303 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 40.000 - 3.000 |
R-factor | 0.23294 |
Rwork | 0.230 |
R-free | 0.28698 |
RMSD bond length | 0.006 |
RMSD bond angle | 0.917 |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 3.110 |
High resolution limit [Å] | 3.000 | 3.000 |
Rmerge | 0.122 | 0.589 |
Number of reflections | 14806 | |
<I/σ(I)> | 15.6 | 2.8 |
Completeness [%] | 99.8 | 99.9 |
Redundancy | 5.9 | 6.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 20 mM HEPES, 1.5 M Li2SO4 |