4WLK
Stationary Phase Survival Protein YuiC from B.subtilis complexed with reaction product
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-07-25 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | H 3 |
| Unit cell lengths | 147.210, 147.210, 37.870 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 42.496 - 2.030 |
| R-factor | 0.1733 |
| Rwork | 0.172 |
| R-free | 0.20510 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4wjt |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.795 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.500 | 2.080 |
| High resolution limit [Å] | 2.030 | 2.030 |
| Rmerge | 0.576 | |
| Number of reflections | 19631 | |
| <I/σ(I)> | 7.5 | 2.2 |
| Completeness [%] | 99.4 | 98.7 |
| Redundancy | 3.8 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 289 | 40% glycerol ethoxylate |
