4WLG
crystal structure of mouse Xyloside xylosyltransferase 1, apo form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-09-20 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.1000 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 88.804, 88.804, 154.324 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 50.000 - 3.000 |
R-factor | 0.2237 |
Rwork | 0.221 |
R-free | 0.27584 |
Structure solution method | SAD |
RMSD bond length | 0.006 |
RMSD bond angle | 0.901 |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.110 |
High resolution limit [Å] | 3.000 | 3.000 |
Rmerge | 0.104 | 0.577 |
Number of reflections | 14732 | |
<I/σ(I)> | 16.6 | 3.6 |
Completeness [%] | 100.0 | 100 |
Redundancy | 10.4 | 10.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 20 mM HEPES, 1.5 M Li2SO4 |