4WLF

Crystal structure of L-malate bound MDH2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2014-05-25
Detector	ADSC QUANTUM 270
Wavelength(s)	0.97934
Spacegroup name	P 21 21 21
Unit cell lengths	60.246, 152.183, 155.034
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.860 - 2.200
R-factor	0.22794
Rwork	0.227
R-free	0.24709
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2dfd
RMSD bond length	0.005
RMSD bond angle	1.026
Refinement software	REFMAC (5.7.0032)

Data quality characteristics

	Overall
Low resolution limit [Å]	34.860
High resolution limit [Å]	2.200
Number of reflections	66329
<I/σ(I)>	54
Completeness [%]	95.9
Redundancy	11.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.2	288	0.1 M Na2HPO4:citric acid, 40% (v/v) polyethylene glycol 300, 10% DMSO