4WLD
High pressure protein crystallography of hen egg white lysozyme at 0.1 MPa
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU FR-E SUPERBRIGHT |
Synchrotron site | Photon Factory |
Beamline | AR-NW12A |
Temperature [K] | 298 |
Detector technology | IMAGE PLATE |
Collection date | 2013-02-12 |
Detector | RIGAKU RAXIS VII |
Wavelength(s) | 1.54178 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 79.197, 79.197, 37.900 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.810 - 1.540 |
R-factor | 0.1498 |
Rwork | 0.148 |
R-free | 0.17882 |
RMSD bond length | 0.023 |
RMSD bond angle | 2.141 |
Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 1.540 |
Number of reflections | 18464 |
<I/σ(I)> | 47.3 |
Completeness [%] | 99.4 |
Redundancy | 7.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | BATCH MODE | 293 | sodium chloride |