4WLD
High pressure protein crystallography of hen egg white lysozyme at 0.1 MPa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E SUPERBRIGHT |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Collection date | 2013-02-12 |
| Detector | RIGAKU RAXIS VII |
| Wavelength(s) | 1.54178 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 79.197, 79.197, 37.900 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.810 - 1.540 |
| R-factor | 0.1498 |
| Rwork | 0.148 |
| R-free | 0.17882 |
| RMSD bond length | 0.023 |
| RMSD bond angle | 2.141 |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 1.540 |
| Number of reflections | 18464 |
| <I/σ(I)> | 47.3 |
| Completeness [%] | 99.4 |
| Redundancy | 7.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | BATCH MODE | 293 | sodium chloride |
