4WLC
Structure of dextran glucosidase with glucose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL38B1 |
Synchrotron site | SPring-8 |
Beamline | BL38B1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-07-03 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 72.989, 83.736, 103.790 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 44.111 - 2.402 |
R-factor | 0.2195 |
Rwork | 0.217 |
R-free | 0.26280 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2zid |
RMSD bond length | 0.003 |
RMSD bond angle | 0.675 |
Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.550 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.781 | |
Number of reflections | 25354 | |
<I/σ(I)> | 10.86 | 2.23 |
Completeness [%] | 99.6 | 99.4 |
Redundancy | 5.39 | 5.42 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 100mM tris-HCl, 18% PEG 6000, 200mM calcium chloride, 5mM alpha-glucosyl fluoride |