4WLC

Structure of dextran glucosidase with glucose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL38B1
Synchrotron site	SPring-8
Beamline	BL38B1
Temperature [K]	100
Detector technology	CCD
Collection date	2014-07-03
Detector	ADSC QUANTUM 315
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	72.989, 83.736, 103.790
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.111 - 2.402
R-factor	0.2195
Rwork	0.217
R-free	0.26280
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2zid
RMSD bond length	0.003
RMSD bond angle	0.675
Refinement software	PHENIX ((phenix.refine: 1.9_1692))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.550
High resolution limit [Å]	2.400	2.400
Rmerge		0.781
Number of reflections	25354
<I/σ(I)>	10.86	2.23
Completeness [%]	99.6	99.4
Redundancy	5.39	5.42

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	100mM tris-HCl, 18% PEG 6000, 200mM calcium chloride, 5mM alpha-glucosyl fluoride