4WL1
Structure of WzzE Polysaccharide Co-polymerase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 85 |
Detector technology | CCD |
Collection date | 2013-08-06 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.98 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 276.849, 246.306, 133.201 |
Unit cell angles | 90.00, 89.99, 90.00 |
Refinement procedure
Resolution | 47.810 - 5.989 |
R-factor | 0.2369 |
Rwork | 0.234 |
R-free | 0.24550 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3b8o |
RMSD bond length | 0.012 |
RMSD bond angle | 2.248 |
Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.810 | 6.200 |
High resolution limit [Å] | 5.990 | 5.900 |
Number of reflections | 43381 | |
<I/σ(I)> | 11.73 | 1.15 |
Completeness [%] | 96.4 | 83.54 |
Redundancy | 3.5 | 2.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 6.5 | 298 | 8% PEG 8000, 0.1M Bis-Tris pH 6.5, 0.2M NaCl, 15% glycerol |