4WL0
Ligand-free structure of human platelet phosphofructokinase in an R-state, crystal form I
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-10-15 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.91841 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 79.343, 178.062, 133.117 |
Unit cell angles | 90.00, 104.45, 90.00 |
Refinement procedure
Resolution | 46.970 - 2.890 |
R-factor | 0.212 |
Rwork | 0.211 |
R-free | 0.23630 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | unpublished low resolution structure |
RMSD bond length | 0.005 |
RMSD bond angle | 1.092 |
Data reduction software | XDS |
Data scaling software | XSCALE (2.5.1) |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.060 | |
High resolution limit [Å] | 2.890 | 8.540 | 2.890 |
Rmerge | 0.077 | 0.019 | 0.573 |
Rmeas | 0.091 | 0.023 | 0.676 |
Total number of observations | 289757 | ||
Number of reflections | 79810 | 3126 | 12688 |
<I/σ(I)> | 13.1 | 52.72 | 2.11 |
Completeness [%] | 99.3 | 98.1 | 98.1 |
Redundancy | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 292 | 0.1 M KCl, 0.025 M MgCl2, 0.05 M Na Cacodylate, 15% iso-Propanol |