4WKJ
Crystallographic Structure of a Dodecameric RNA-DNA Hybrid
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | RIGAKU SATURN 92 |
Wavelength(s) | 1.54 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 46.649, 44.459, 83.346 |
Unit cell angles | 90.00, 105.27, 90.00 |
Refinement procedure
Resolution | 26.270 - 2.800 |
R-factor | 0.23874 |
Rwork | 0.238 |
R-free | 0.24204 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1zbi |
RMSD bond length | 0.009 |
RMSD bond angle | 1.698 |
Data scaling software | d*TREK |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0085) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 26.270 | 2.900 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.086 | 0.492 |
Number of reflections | 8314 | |
<I/σ(I)> | 13.4 | 2.7 |
Completeness [%] | 99.9 | 99.9 |
Redundancy | 7.3 | 7.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 298 | 20% PEG 3350, 0.2 M magnesium formate, 10% 2-Methyl-2,4-pentanediol |