4WK4
Metal Ion and Ligand Binding of Integrin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-02-23 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.97934 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 57.520, 112.160, 169.540 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.597 - 2.500 |
R-factor | 0.1892 |
Rwork | 0.187 |
R-free | 0.23580 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.005 |
RMSD bond angle | 0.985 |
Data scaling software | XDS |
Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 47.600 |
High resolution limit [Å] | 2.500 |
Number of reflections | 38535 |
<I/σ(I)> | 7.17 |
Completeness [%] | 99.3 |
Redundancy | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 293 | 0.1 M HEPES 7.2, 16% PEG 6000 |