4WK1
Crystal structure of Staphylococcus aureus PstA in complex with c-di-AMP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-09-10 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 0.99998 |
Spacegroup name | H 3 2 |
Unit cell lengths | 68.100, 68.100, 135.740 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 44.517 - 1.980 |
R-factor | 0.192 |
Rwork | 0.188 |
R-free | 0.22880 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3m05 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.201 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.030 |
High resolution limit [Å] | 1.980 | 1.980 |
Number of reflections | 8523 | |
<I/σ(I)> | 23.6 | 2 |
Completeness [%] | 97.7 | 81.4 |
Redundancy | 16.6 | 5.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 6 % w/v PEG 8000, 0.1 M HEPES pH 7.0, 0.2 M calcium-acetate |