4WJW

Crystal Structure of the Chs5-Chs6 Exomer Cargo Adaptor Complex Bound to portion of Chs3

Replaces:  4U9T

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE A1
Synchrotron site	CHESS
Beamline	A1
Temperature [K]	100
Detector technology	CCD
Collection date	2012-03-16
Detector	ADSC QUANTUM 210
Wavelength(s)	.9767
Spacegroup name	P 63 2 2
Unit cell lengths	218.613, 218.613, 137.848
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.650 - 2.590
R-factor	0.181
Rwork	0.180
R-free	0.21100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4GNS
Refinement software	PHENIX ((PHENIX.REFINE: 1.8.4_1496))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.650	2.683
High resolution limit [Å]	2.590	2.590
Number of reflections	59936
<I/σ(I)>	19.45
Completeness [%]	99.0	99
Redundancy	12.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.5	293	0.3 M AMMONIUM SULFATE, 0.1 M CITRIC ACID