4WJU
Crystal structure of Rsa4 from Saccharomyces cerevisiae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-11-22 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.0 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 96.870, 108.470, 261.910 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.609 - 2.800 |
| R-factor | 0.206 |
| Rwork | 0.203 |
| R-free | 0.24610 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4wjs |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.861 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.610 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.094 | 0.470 |
| Number of reflections | 33508 | |
| <I/σ(I)> | 7.68 | 2.25 |
| Completeness [%] | 97.3 | 98.6 |
| Redundancy | 1.9 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 3.5M NaHCO2, 2.25M NH4Ac |
