4WJO
Crystal Structure of SUMO1 in complex with PML
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-10-11 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 38.388, 47.253, 63.908 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 26.470 - 1.460 |
| R-factor | 0.158 |
| Rwork | 0.157 |
| R-free | 0.18380 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2uyz |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.363 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine: dev_1555)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.540 |
| High resolution limit [Å] | 1.460 | 1.460 |
| Rmerge | 0.092 | 0.597 |
| Number of reflections | 20770 | |
| <I/σ(I)> | 11.6 | 2.7 |
| Completeness [%] | 99.2 | 95.4 |
| Redundancy | 6.8 | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 100mM sodium cacodylate pH6.5, 16% PEG3350, 10mM calcium chloride |
