4WJ9
Structure of Human apo ALDH1A1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-11-10 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.03 |
| Spacegroup name | P 4 2 2 |
| Unit cell lengths | 109.297, 109.297, 83.236 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 109.300 - 1.740 |
| R-factor | 0.1863 |
| Rwork | 0.185 |
| R-free | 0.21562 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.193 |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 109.300 | |
| High resolution limit [Å] | 1.740 | 1.740 |
| Rmerge | 0.056 | 0.590 |
| Number of reflections | 48862 | |
| <I/σ(I)> | 31.5 | 3.9 |
| Completeness [%] | 99.0 | 97 |
| Redundancy | 9.6 | 8.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 7 | 300 | PEG3350, sodium chloride, 100 mM Bis Tris Buffer, ytterbium chloride, sitting drop |
