4WJ4
Crystal structure of non-discriminating aspartyl-tRNA synthetase from Pseudomonas aeruginosa complexed with tRNA(Asn) and aspartic acid
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL44XU |
Synchrotron site | SPring-8 |
Beamline | BL44XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-06-02 |
Detector | RAYONIX MX225HE |
Wavelength(s) | 0.9 |
Spacegroup name | P 41 2 2 |
Unit cell lengths | 157.913, 157.913, 146.260 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.478 - 3.294 |
R-factor | 0.1912 |
Rwork | 0.189 |
R-free | 0.22440 |
RMSD bond length | 0.003 |
RMSD bond angle | 0.615 |
Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 47.260 |
High resolution limit [Å] | 3.290 |
Number of reflections | 28534 |
<I/σ(I)> | 14.53 |
Completeness [%] | 99.8 |
Redundancy | 14.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.7 | 298 | Trisodium citrate |