Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-06-21 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.979 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 125.890, 58.672, 90.147 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.336 - 2.850 |
R-factor | 0.2294 |
Rwork | 0.227 |
R-free | 0.28190 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3LFX |
RMSD bond length | 0.007 |
RMSD bond angle | 1.121 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: dev_1702)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.340 | 3.000 |
High resolution limit [Å] | 2.850 | 2.850 |
Rmerge | 0.130 | 0.640 |
Number of reflections | 15944 | |
<I/σ(I)> | 9.7 | 3.1 |
Completeness [%] | 98.8 | |
Redundancy | 4.7 | 4.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 3.5 | 298 | 19% PEG 3350, 0.1M CITRIC ACETATE |