4WIT
TMEM16 lipid scramblase in crystal form 2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-05-10 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 115.940, 127.240, 180.110 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 14.997 - 3.400 |
R-factor | 0.2498 |
Rwork | 0.248 |
R-free | 0.29170 |
Structure solution method | SAD |
RMSD bond length | 0.002 |
RMSD bond angle | 0.699 |
Data reduction software | XDS |
Data scaling software | SCALEPACK |
Phasing software | SHELX |
Refinement software | PHENIX ((phenix.refine: dev_1760)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.500 |
High resolution limit [Å] | 3.400 | 3.400 |
Rmerge | 0.065 | 1.470 |
Number of reflections | 36952 | |
<I/σ(I)> | 20 | 1.7 |
Completeness [%] | 98.9 | 87.9 |
Redundancy | 9.6 | 8.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 277 | 21-23% PEG400, 50 mM HEPES pH 7.4, 100 mM Ammonium sulfate |