4WIS
Crystal structure of the lipid scramblase nhTMEM16 in crystal form 1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-06-13 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 0.9797 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 96.480, 113.690, 235.650 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 14.984 - 3.300 |
R-factor | 0.2399 |
Rwork | 0.237 |
R-free | 0.28470 |
Structure solution method | SAD |
RMSD bond length | 0.003 |
RMSD bond angle | 0.736 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | SHELX |
Refinement software | PHENIX ((phenix.refine: dev_1760)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.400 |
High resolution limit [Å] | 3.300 | 3.300 |
Rmerge | 1.235 | |
Number of reflections | 39477 | |
<I/σ(I)> | 20.1 | 2.6 |
Completeness [%] | 88.1 | 98.8 |
Redundancy | 12.7 | 12.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 277 | 21-23% PEG 400, 100 mM Capso pH 9.4, 100 mM MgCl2, 100 mM NaCl |