4WIP

DIX domain of human Dvl2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E+ SUPERBRIGHT
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2014-08-22
Detector	RIGAKU RAXIS HTC
Wavelength(s)	1.54178
Spacegroup name	P 21 21 21
Unit cell lengths	56.300, 84.820, 98.260
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	64.210 - 2.691
R-factor	0.22351
Rwork	0.222
R-free	0.24816
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	1.807
Data reduction software	MOSFLM
Data scaling software	SCALA (3.3.21)
Phasing software	PHASER
Refinement software	REFMAC (5.8.0085)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	98.260	42.513	2.840
High resolution limit [Å]	2.691	8.510	2.690
Rmerge		0.096	0.480
Rmeas	0.170
Rpim	0.095	0.065	0.331
Total number of observations	41191	1310	5815
Number of reflections	13481
<I/σ(I)>	5.9	9	2.1
Completeness [%]	99.4	95.3	99.9
Redundancy	3.1	2.8	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	292	grew in PEG400 (cryo condition) over night