4WIM
Crystal Structure of the GMP Synthetase from Plasmodium falciparum
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-10-06 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97239 |
Spacegroup name | P 1 |
Unit cell lengths | 43.453, 68.548, 102.080 |
Unit cell angles | 101.11, 99.17, 93.71 |
Refinement procedure
Resolution | 49.305 - 3.600 |
R-factor | 0.2821 |
Rwork | 0.281 |
R-free | 0.29270 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3uow |
RMSD bond length | 0.035 |
RMSD bond angle | 3.448 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 98.600 | 3.960 |
High resolution limit [Å] | 3.600 | 3.600 |
Rmerge | 0.640 | |
Number of reflections | 13123 | |
<I/σ(I)> | 7.2 | 1.34 |
Completeness [%] | 88.8 | 84 |
Redundancy | 1.7 | 1.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 292 | 20% (w/v) PEG 3350, 0.2 M Diammonium tartrate and 5 mM NaCl |