4WIL
Crystal structure of DCoH2 S51T
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-BM |
Synchrotron site | APS |
Beamline | 22-BM |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2011-08-17 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 1.00 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 57.729, 57.729, 115.064 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 24.997 - 1.360 |
R-factor | 0.1587 |
Rwork | 0.158 |
R-free | 0.17460 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ru0 |
RMSD bond length | 0.006 |
RMSD bond angle | 1.045 |
Refinement software | PHENIX ((phenix.refine: 1.6.4_486)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 1.380 |
High resolution limit [Å] | 1.360 | 1.360 |
Rmerge | 0.953 | |
Number of reflections | 48837 | |
<I/σ(I)> | 58.1 | 2.98 |
Completeness [%] | 97.8 | 96.4 |
Redundancy | 10.7 | 9.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 7.5 | 291 | 0.1M Tris, 8% PEG 8,000 mixing 0.5 ul protein and 1 ul reservoir |