4WIJ
HUMAN SPLICING FACTOR, CONSTRUCT 1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
Synchrotron site | Australian Synchrotron |
Beamline | MX2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-10-17 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9537 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 66.580, 66.580, 398.020 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.080 - 3.490 |
R-factor | 0.275 |
Rwork | 0.272 |
R-free | 0.33500 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 0.880 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | BUSTER (2.10.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.080 | 3.670 |
High resolution limit [Å] | 3.480 | 3.480 |
Rmerge | 0.100 | 0.307 |
Number of reflections | 12337 | |
<I/σ(I)> | 10.8 | 4.3 |
Completeness [%] | 99.7 | 98.1 |
Redundancy | 6.8 | 6.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 26% (V/V) 1,4 DIOXANE, 0.05M MOPS, 50 MM MAGNESIUM CHLORIDE HEXAHYDRATE, 1 MM SPERMINE, PH 7.0, VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 293K |