4WII
HUMAN SPLICING FACTOR, CONSTRUCT 3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-10-15 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.9686 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 63.447, 67.611, 119.528 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.520 - 2.050 |
| R-factor | 0.201 |
| Rwork | 0.199 |
| R-free | 0.23500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.040 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.610 | 2.160 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Number of reflections | 32938 | |
| <I/σ(I)> | 13.1 | 2.1 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 1.9 M AMMONIUM SULFATE, 0.1 M TRIS, 20% ETHYLENE GLYCOL (V/V), PH 8.5, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K |
