4WIH

Crystal structure of cAMP-dependent Protein Kinase A from Cricetulus griseus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-03-21
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.918409
Spacegroup name	P 21 21 21
Unit cell lengths	58.276, 72.949, 109.351
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.874 - 1.139
R-factor	0.178
Rwork	0.177
R-free	0.19140
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1yds
RMSD bond length	0.006
RMSD bond angle	1.013
Data reduction software	XDS ((xdsapp 1.02))
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.9_1692))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.530	1.210
High resolution limit [Å]	1.139	1.139
Number of reflections	169712
<I/σ(I)>	17.59	2.32
Completeness [%]	99.7	98.7
Redundancy	6.49	6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6.5	277	Reservoir: 20-25% Methanol Drop: 25mM MesBisTris buffer pH6.5, 50mM LiCl, 1mM PKI, 0.35mM PKA