4WIC
Immediate-early 1 protein (IE1) of rhesus macaque cytomegalovirus
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-09-16 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.9184, 1.0402, 1.0372, 1.0346, 0.9797 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 57.950, 278.550, 61.620 |
| Unit cell angles | 90.00, 90.90, 90.00 |
Refinement procedure
| Resolution | 19.935 - 2.850 |
| R-factor | 0.2142 |
| Rwork | 0.212 |
| R-free | 0.25940 |
| Structure solution method | MAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.438 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHARP |
| Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.920 |
| High resolution limit [Å] | 2.850 | 2.850 |
| Number of reflections | 45137 | |
| <I/σ(I)> | 16.9 | |
| Completeness [%] | 99.5 | 99.7 |
| Redundancy | 3.7 | 1.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293.15 | Reservoir 700 microL: 15 % (w/v) PEG 3350, 400 mM magnesium formate Drop ratio: 1 to 2 microL protein solution (20 mg/mL) plus 1 microL of reservoir solution supplemented with microseeds |
