4WIA

Crystal structure of flagellar accessory protein FlaH from Methanocaldococcus jannaschii

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL38B1
Synchrotron site	SPring-8
Beamline	BL38B1
Temperature [K]	100
Detector technology	CCD
Collection date	2014-05-27
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97886, 0.97944
Spacegroup name	P 31 2 1
Unit cell lengths	130.960, 130.960, 89.081
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	50.000 - 2.200
R-factor	0.20687
Rwork	0.206
R-free	0.23296
Structure solution method	MAD
RMSD bond length	0.015
RMSD bond angle	1.854
Refinement software	REFMAC (5.8.0073)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.200
Number of reflections	45022
<I/σ(I)>	11.9
Completeness [%]	100.0
Redundancy	10.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	283	0.1 M HEPES, 0.65 M Rb2SO4