4WI3

Structural mapping of the human IgG1 binding site for FcRn: hu3S193 Fc mutation I253A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	CCD
Collection date	2010-03-16
Detector	ADSC QUANTUM 210
Wavelength(s)	0.954
Spacegroup name	P 21 21 21
Unit cell lengths	49.129, 75.908, 142.368
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.823 - 2.703
R-factor	0.2236
Rwork	0.216
R-free	0.29640
RMSD bond length	0.007
RMSD bond angle	1.151
Refinement software	PHENIX ((phenix.refine: 1.7.3_928))

Data quality characteristics

	Overall
Low resolution limit [Å]	30.000
High resolution limit [Å]	2.700
Number of reflections	15044
<I/σ(I)>	9.4
Completeness [%]	99.0
Redundancy	5.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	291	PEG 6000, 0.1 M MES, 25% v/v ethylene glycol