4WHY
Structure of the Hepatitis C virus envelope glycoprotein E2 antigenic region 412-423 bound to the broadly neutralizing antibody 3/11, P21 crystal form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-03-20 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97902 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 64.760, 205.510, 69.020 |
| Unit cell angles | 90.00, 103.18, 90.00 |
Refinement procedure
| Resolution | 47.970 - 2.620 |
| R-factor | 0.2067 |
| Rwork | 0.204 |
| R-free | 0.25800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.250 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Refinement software | BUSTER (2.11.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.760 |
| High resolution limit [Å] | 2.620 | 2.620 |
| Rmerge | 0.444 | |
| Number of reflections | 51028 | |
| <I/σ(I)> | 9.9 | 1.6 |
| Completeness [%] | 97.0 | 87.5 |
| Redundancy | 3.2 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | 100 mM TRIS, 66% MPD |
