4WHU
BROMO domain of CREB binding protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-11-29 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 25.284, 43.317, 51.387 |
| Unit cell angles | 90.00, 98.68, 90.00 |
Refinement procedure
| Resolution | 32.960 - 2.110 |
| R-factor | 0.2101 |
| Rwork | 0.208 |
| R-free | 0.26120 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3dvw |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.950 |
| Refinement software | BUSTER (2.11.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.960 | 2.130 |
| High resolution limit [Å] | 2.110 | 2.100 |
| Rmerge | 0.069 | 0.498 |
| Number of reflections | 6356 | |
| <I/σ(I)> | 24.6 | 4.3 |
| Completeness [%] | 99.3 | 90.9 |
| Redundancy | 3.9 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | obtained through screen with Hampton Research kits (HT and Index) |
