4WHJ
Myxovirus Resistance Protein 2 (MxB)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-02-09 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9792 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 53.185, 80.782, 183.666 |
Unit cell angles | 90.00, 95.73, 90.00 |
Refinement procedure
Resolution | 43.929 - 3.200 |
R-factor | 0.2667 |
Rwork | 0.265 |
R-free | 0.29930 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3szr |
RMSD bond length | 0.002 |
RMSD bond angle | 0.630 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 3.260 |
High resolution limit [Å] | 3.200 | 8.670 | 3.200 |
Rmerge | 0.089 | 0.040 | |
Total number of observations | 132310 | ||
Number of reflections | 24322 | ||
<I/σ(I)> | 8.9 | ||
Completeness [%] | 94.8 | 97 | 89.9 |
Redundancy | 5.4 | 6 | 5.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH | 298 | 0.1 M MES, 5% PEG4000, 10% 2-Propanol, 0.05 M magnesium chloride |