4WGY
Crystal Structure of Cytochrome c' from Alcaligenes xylosoxidans NCIMB 11015 at pH 10.4
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
Synchrotron site | Photon Factory |
Beamline | AR-NW12A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-06-10 |
Detector | ADSC QUANTUM 210r |
Wavelength(s) | 0.7500 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 52.666, 52.666, 183.584 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 45.610 - 1.480 |
R-factor | 0.14002 |
Rwork | 0.138 |
R-free | 0.17253 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1e83 |
RMSD bond length | 0.026 |
RMSD bond angle | 2.770 |
Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 1.480 |
Number of reflections | 26188 |
<I/σ(I)> | 64.1 |
Completeness [%] | 99.6 |
Redundancy | 20.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 10.4 | 293 | 24 % PEG4000, 100 mM CAPS, 50 mM magnesium chloride |