4WG7
Room-temperature crystal structure of lysozyme determined by serial synchrotron crystallography using a nano focused beam.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID13 |
| Synchrotron site | ESRF |
| Beamline | ID13 |
| Temperature [K] | 293 |
| Detector technology | CCD |
| Collection date | 2014-04-19 |
| Detector | CUSTOM-MADE |
| Wavelength(s) | 0.832 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 77.990, 77.990, 38.510 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.995 - 1.700 |
| R-factor | 0.2055 |
| Rwork | 0.204 |
| R-free | 0.23890 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1jln |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.935 |
| Data reduction software | CrystFEL |
| Data scaling software | CrystFEL |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.140 | 1.780 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 1.697 | |
| Number of reflections | 13570 | |
| <I/σ(I)> | 6.8 | 0.9 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 787 | 430 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 293 | 50mM Sodium acetate pH 4.5, 16% PEG 4K, 3.5M NaCl |
