4WG0
Crystal structure of a tridecameric superhelix
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-11-17 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9722 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 95.869, 37.784, 101.430 |
| Unit cell angles | 90.00, 96.84, 90.00 |
Refinement procedure
| Resolution | 45.158 - 1.820 |
| R-factor | 0.1866 |
| Rwork | 0.185 |
| R-free | 0.21760 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | ideal alpha-helix |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.525 |
| Data reduction software | DENZO |
| Data scaling software | SADABS |
| Phasing software | Arcimboldo |
| Refinement software | PHENIX ((phenix.refine: dev_1769)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.200 | 1.890 |
| High resolution limit [Å] | 1.820 | 1.820 |
| Rmerge | 0.050 | 0.558 |
| Number of reflections | 32046 | |
| <I/σ(I)> | 15.1 | 3 |
| Completeness [%] | 97.5 | 93.1 |
| Redundancy | 4.1 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 3.5 | 298 | 2M ammonium sulfate, 5-10% glycerol, 0.1 M citric acid pH 3.5 |
