4WFY
Coxsackievirus B3 Polymerase - F232L Mutant - AmSO4 Crystal Form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 4.2.2 |
Synchrotron site | ALS |
Beamline | 4.2.2 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2013-06-12 |
Detector | NOIR-1 |
Wavelength(s) | 1.00 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 74.360, 74.360, 288.050 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 52.580 - 2.060 |
R-factor | 0.1886 |
Rwork | 0.187 |
R-free | 0.22010 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.057 |
Data scaling software | XSCALE |
Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 52.600 |
High resolution limit [Å] | 2.060 |
Number of reflections | 710311 |
<I/σ(I)> | 18.65 |
Completeness [%] | 99.3 |
Redundancy | 13.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7.5 | 289 | 0.085 M Tris, 1.275 M Ammonium Sulfate , 25% Glycerol |