4WFX

Coxsackievirus B3 Polymerase - F232L Mutant - NaCl Crystal Form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2013-06-12
Detector	NOIR-1
Wavelength(s)	1.00
Spacegroup name	P 43 21 2
Unit cell lengths	74.360, 74.360, 288.050
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	52.580 - 1.808
R-factor	0.1984
Rwork	0.198
R-free	0.23250
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3ddk
RMSD bond length	0.007
RMSD bond angle	1.063
Data scaling software	XSCALE
Refinement software	PHENIX ((phenix.refine: 1.8.2_1309))

Data quality characteristics

	Overall
Low resolution limit [Å]	52.600
High resolution limit [Å]	1.800
Number of reflections	74782
<I/σ(I)>	18.97
Completeness [%]	99.4
Redundancy	12.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.5	289	0.085 M HEPES, 3.655 M NaCl, 15% Glycerol