4WFV

Bovine allergen Bos d 2 in the monoclinic space group C2.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	CCD
Collection date	2012-06-15
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.011
Spacegroup name	C 1 2 1
Unit cell lengths	125.940, 36.590, 36.470
Unit cell angles	90.00, 95.86, 90.00

Refinement procedure

Resolution	25.603 - 1.400
R-factor	0.1971
Rwork	0.196
R-free	0.21350
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1bj7
RMSD bond length	0.006
RMSD bond angle	1.081
Data reduction software	XDS
Data scaling software	XSCALE
Refinement software	PHENIX ((phenix.refine: 1.7_650))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.500
High resolution limit [Å]	1.400	1.400
Rmerge	0.044	0.368
Number of reflections	31792
<I/σ(I)>	22.3	4.2
Completeness [%]	96.9	88.8
Redundancy	3.5	2.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.4	293	2.5 M 1,6-hexanediol, 0.1 M sodium citrate